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Chemistry: Molecular Biology And Microbiology > Animal Cell, Per Se (e.g., Cell Lines, Etc.); Composition Thereof; Process Of Propagating, Maintaining Or Preserving An Animal Cell Or Composition Thereof; Process Of Isolating Or Separating An Animal Cell Or Composition Thereof; Process Of Preparing A Composition Containing An Animal Cell; Culture Media Therefore Animal Cell, Per Se (e.g., Cell Lines, Etc.); Composition Thereof; Process Of Propagating, Maintaining Or Preserving An Animal Cell Or Composition Thereof; Process Of Isolating Or Separating An Animal Cell Or Composition Thereof; Process Of Preparing A Composition Containing An Animal Cell; Culture Media ThereforeAnimal Cell, Per Se (e.g., Cell Lines, Etc.); Composition Thereof; Process Of Propagating, Maintaining Or Preserving An Animal Cell Or Composition Thereof; Process Of Isolating Or Separating An Animal Cell Or Composition Thereof; Process Of Preparing A Composition Containing An Animal Cell; Culture Media Therefore patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.04/19/07 - 20070087434 - Antiviral artificial cell An antiviral artificial cell includes: an artificial cytoskeleton, an artificial cytomembrane wrapping the artificial cytoskeleton, and a nanoparticle rotatably retained on the artificial cytomembrane and having a surface for capturing a virus. ... 04/12/07 - 20070082395 - Production of normal human osteoclasts The present invention provides a method of producing a cell population enriched for osteoclasts in which bone marrow mononuclear cells (BMNC) are cultured in a medium supplemented with growth factors, cytokines, or a combination thereof for at least 7 days to obtain colony-forming units of granulocytes and macrophages (CFU-GM); CFU-GM ... 04/12/07 - 20070082394 - Cell culture media, kits and methods of use Albumin-supplemented and xenogeneic product-free cell culture media, cell culture media supplements, and cell culture media kits for the support of primary culture of normal non-hematopoietic cells of mesodermal origin suitable for both research and clinical applications. ... 04/12/07 - 20070082393 - Polymer coated nanofibrillar structures and methods for cell maintenance and differentiation The invention provides cell-adherent polymeric coatings for articles having a nanofibrillar structure. The coatings include a synthetic, non-biodegradable polymer having at least one pendent amine group, wherein the polymer is covalently immobilized on the article via latent reactive groups. The invention also provides methods for the long term culturing of ... 04/12/07 - 20070082392 - Modified erythrocytes and uses thereof The present invention provides modified erythrocytes which comprise viral receptor proteins capable of mediating entry of respective viruses into the modified erythrocytes. The present invention also provides methods of using the modified erythrocytes for the treatment or prevention of viral infections. In one embodiment, the modified erythrocytes of the present ... 04/05/07 - 20070077652 - Methods of ex vivo progenitor and stem cell expansion by co-culture with mesenchymal cells Methods of ex-vivo expansion and at the same time inhibiting differentiation of stem cells by co-culture with mesenchymal cells, transplantable populations of renewable progenitor and stem cells expanded thereby, and their uses in therapeutic applications. ... 04/05/07 - 20070077651 - Peptides for enhanced cell attachment and growth The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created ... 04/05/07 - 20070077650 - Rapid production of high titer virus The invention includes methods of producing viral particles which include introducing into avian cells a nucleotide sequence encoding a replication deficient retroviral vector and introducing into the avian cells nucleotide sequences encoding products required for replication of the replication deficient retroviral vector under the control of a promoter that is ... 04/05/07 - 20070077649 - Transplantable cell growth niche and related compositions and methods Provided is a method of culturing a successor cell from a precursor cell comprising co-culturing a precursor cell such as, without limitation, a human embryonic stem cell or a neuronal stem cell with an adult mesenchymal stem cell. In one embodiment, the adult mesenchymal stem cell is derived from adipose ... 03/29/07 - 20070072291 - Dedifferentiated, programmable stem cells of monocytic origin, and their production and use The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for ... 03/22/07 - 20070065940 - Method of preparing basement membrane, method of constructing basement membrane specimen, reconstituted artificial tissue using the basement membrane specimen and process for producing the same The object of the present invention is to provide the following: a method for preparing a basement membrane which is extracellular matrices having a function to control morphology, differentiation, proliferation, motility, function expression and the like of cells; a method for constructing a specimen of a basement membrane; a process ... 03/22/07 - 20070065939 - Systems for detection and production of respiratory, herpes and enteric viruses The present invention generally relates to the field of diagnostic microbiology, and, more particularly to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial ... 03/22/07 - 20070065938 - Perivascular mesenchymal precursor cells Mesenchymal precursors cells have been isolated from perivascular niches from a range of tissues utilizing a perivascular marker. A new mesenchymal precursor cell phenotype is described characterized by the presence of the perivascular marker 3G5, and preferably also alpha smooth muscle actin together with early developmental markers such as MUC ... 03/15/07 - 20070059821 - Method for preparing cell cultures from biological specimens for chemotherapeutic and other assays An improved method for preparing a cell culture is disclosed. The method includes culturing a multicellular tissue explant in the presence of growth medium that is substantially free of enzymes capable of digesting the explant and, subsequently, removing the explant at a predetermined time. ... 03/08/07 - 20070054397 - Adult cardiac uncommitted progenitor cells The invention provides for adult cardiac uncommitted progenitor cells (UPCs), methods of making such cells, and methods of using such cells in the treatment, repair and/or regeneration of damaged cardiac tissue. ... 03/08/07 - 20070054396 - Cell line and methods for determining viral titer The present invention relates to cells, methods, compositions and kits for determining the concentration of virus in a stock, i.e., determining the titer of a viral stock. ... 03/01/07 - 20070048863 - Computerized factorial experimental design and control of reaction sites and arrays thereof Computer-facilitated design of large-scale, multi-factorial cell culture experiments and the like, and control of reaction sites and/or arrays of reaction sites to perform such experiments using automated devices. In certain cases, the invention is directed to controlling a plurality of cell culture experiments, e.g., using an automated cell culture device. ... 02/22/07 - 20070042490 - Apparatus and method for tissue engineering A bioreactor system includes a housing and a hydrostatic loading module. The housing includes a chamber with an inlet port and an outlet port. The inlet and outlet ports allowing the chamber to be continuously perfused with a culture medium while chamber is hydrostatically loaded. ... 02/15/07 - 20070037280 - Method for generating primate trophoblasts The first method to cause a culture of human and other primate stem cells to directly and uniformly differentiate into a committed cell lineage is disclosed. Treatment of primate stem cells with a single protein trophoblast induction factor causes the cells to transform into human trophoblast cells, the precursor cells ... 02/15/07 - 20070037279 - Cell culture system The present invention provides a novel apparatus to grow cells where the cultivation chamber (1) is partially filled with liquid cultivation medium and cells. Mixing and aeration is achieved by generating intermittently one single large gas bubble (6) at the bottom of the column bioreactor, the single large bubble width ... 02/08/07 - 20070031965 - Anti-pathogen treatments Chimeric molecules that contain at least one pathogen-detection domain and at least one effector domain, and their methods of use in preventing or treating a pathogen infection in a cell or organism are described. The pathogen-detection domain and effector domain of the chimeric molecules are domains not typically found in ... 02/08/07 - 20070031964 - Myeloma cell culture in transferrin-free low iron medium The present invention relates to a method for culturing mammalian cells in a culture medium which is transferrin free and which contains no lipophilic or synthetic nitrogen-containing chelators. Also provided is the use of the medium and a process for providing a mammalian product by culturing cells capable of producing ... 02/01/07 - 20070026519 - Devices and methods for pharmacokinetic-based cell culture systems Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device. ... 02/01/07 - 20070026518 - Controlling stem cell destiny with tunable matrices The present invention provides a class of interpenetrating polymeric networks (IPNs) and semi-interpenetrating polymeric networks (sIPNs) which include a covalently grafted growth factor or differentiation factor for a stem cell. ... 02/01/07 - 20070026517 - Method and bioreactor for the cultivation and stimulation of three-dimensional, vitally and mechanically reistant cell transplants A process and a bioreactor for the manufacturing of three-dimensional, vital and mechanically-resistant cell cultures that can be cultivated and stimulated within a short time of each other or simultaneously. The bioreactor has a basic body connected to a reactor lock so that it is pressure proof and sterile, this ... 01/25/07 - 20070020756 - Methods of culturing, storing, and inducing differentiation in cells, instrument for use in the methods, method of using the instrument, and medical biomaterial The present invention provides methods of culturing or storing cells for a prolonged period while suppressing a loss of functions of the cells during storage, by applying centrifugal force or pressure such as hydraulic pressure to the cells to form compact bodies, particularly aggregates, in which a state of high ... 01/25/07 - 20070020755 - Stage specific follicle maturation systems Improved three dimensional culture systems for the maturation and development of individual follicles and oocytes. The present invention optimizes preantral two-layer secondary follicle growth and maturation, preantral multilayer secondary follicle growth and maturation, and oocyte developmental competence by encapsulating individual follicles into polymeric hydrogel beads having optimal concentrations of polysaccharide. ... 01/25/07 - 20070020754 - Cell handling device, human tissue regeneration composition, and human tissue regeneration method The main objects of the present invention, which relates to regenerative medical treatments, are to enable (i) storage and conveyance of harvested or cultured cells without contamination occuring (ii) simple injection of the cells into a living body. To achieve these objects, cells harvested from a living body, or cells ... 01/18/07 - 20070015277 - Substrate for cell transfer A main object of the present invention is to provide a cell transfer substrate capable of transplanting cells, maintaining a pattern as it is, on a living body tissue or the like, even when: the size of the cell sheet is extremely small; the cells are cultured sparsely; the cells ... 01/11/07 - 20070010008 - Ex vivo expansion of primary animal cells An ex vivo method of expanding animal cells whose differentiation state is controllable by modulating TGF-β signaling includes the steps of: (a) providing an animal subject having cells with a first phenotype; (b) isolating the cells from the animal subject; (c) placing the cells in an ex vivo culture system ... 01/04/07 - 20070004037 - Methods and compositions for culturing keratinocytes Methods, compositions, and kits are disclosed for culturing keratinocytes and for the treatment of wounds and burns, as well as cosmetic compositions. The methods and compositions include the use of conditioned medium from the culture of keratinocytes, where the medium may be used to further culture keratinocytes or used in ... 01/04/07 - 20070004036 - Methods and compositions for keratinocyte culture The invention encompasses composition and methods for cell culture and for therapeutic and cosmetic use. The compositions and methods utilize collagenase, e.g., bacterial collagenase, other isolated collagenase, or synthetic collagenase, e.g., recombinant collagenase. One form of collagenase that can be used in some embodiments of the invention is matrix metalloproteinase-1. ... 01/04/07 - 20070004035 - Gene therapy methods and cell growth and cell transplant devices for use therein An artificial tendon, ligament, bone and skin panel are provided, made of biocompatible materials and capable of being seeded and eventually coated with selected cell types. Seeded or coated artificial tendon, ligament, bone or skin panels can be shaped and implanted in mammals. Implantable cell growth cages may be seeded ... 12/28/06 - 20060292692 - Potentially regenerative cells and functional tissue-organs in vitro Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil ... 12/28/06 - 20060292691 - Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (sina) The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA ... 12/28/06 - 20060292690 - Method of making cell growth surface The present invention discloses a three-dimensional porous growth surface made from polysaccharide material, especially the alginic acid, to enhance cell growth surface, promote cell adherence, immobilization and propagation, maintain surface structure integrity, enable programmable degradation, and thus increase cellular production. The present invention teaches several methods: a method to enhance ... 12/28/06 - 20060292689 - Car receptor as a mediator of migratory cell chemotaxis and/or chemokinesis This invention relates to methods and compositions for modulating movement of eukaryotic cells with migratory capacity. More specifically, the invention relates to methods and compositions for modulating movement of CaR receptor expressing cells of hematopoietic, neural, epithelial, endothelial, or mesenchymal origin, in a specific site in a subject. The foregoing ... 12/28/06 - 20060292688 - System and sequential culture media for in vitro fertilization Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture ... 12/21/06 - 20060286669 - Isolation of multi-lineage stem cells The present application discloses a method of manipulating a biological sample of cells, which includes multi-lineage stem cells, progenitor cells, other marrow stromal cells: allowing the sample of cells to settle in a container; transferring supernatant from the container to another container; and isolating cells from the supernatant, which has ... 12/21/06 - 20060286668 - Animal-cell culture media comprising non-animal or plant-derived nutrients The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation or animal cells. The media comprise at least one nutrient of non-animal derivation, such as at least one plant peptide and/or at least one non-animal or plant lipid and/or fatty acid. The ... 12/21/06 - 20060286667 - Methods for detection and production of influenza viruses The present invention generally relates to the field of diagnostic microbiology, and, more particularly; to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial ... 12/21/06 - 20060286666 - Methods and apparatuses for culturing stem cell using biomaterial shell Disclosed are methods and apparatuses for culturing stem cell using biomaterial shell, e.g. fertilized zebrafish chorions, which may induce formation of embryonic bodies, cell proliferation, and cell differentiation to other types effectively inside the chorions without adding any inducing agents or cell differentiation stimulants. There is provided an apparatus for ... 12/14/06 - 20060281176 - In-vitro fertilization systems with spermatozoa separated into x-chromosome and y-chromosome bearing populations An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) ... 12/14/06 - 20060281175 - Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (sina) The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA ... 12/14/06 - 20060281174 - Methods for generating insulin-producing cells This invention relates to methods for generating insulin-producing cells. In particular, the present invention relates to methods for generating insulin-producing cells or precursors thereof in vitro or in vivo by employing mesenchymal stem cells and pancreatic cells. Insulin-producing cells or precursors thereof created in accordance with the present methods, as ... 12/14/06 - 20060281173 - Composition for coating support for preparation of cell sheet support for preparation of cell sheet and process for producing cell sheet Disclosed are a method for manufacturing a cell sheet, comprising culturing cells on a fibrin-coated surface of a substrate until the cells reach confluency, continuing the cultivation of the cells for a sufficient time period to cause the degradation of fibrin at the bottom of the cells, and detaching the ... 12/07/06 - 20060275898 - Compositions and methods for making embryonic stem cells The invention relates to cell proliferation, cell differentiation, male infertility, male fertility and to compositions and methods involved therein. ... 11/30/06 - 20060270033 - Composition containing gamete or embryo and animal white yolk and the use thereof A composition that contains a gamete or an embryo and animal white yolk is disclosed. Also disclosed are applications of the composition. ... 11/30/06 - 20060270032 - Microscale micropatterened engineered in vitro tissue The disclosure provides an in vitro culture systems. The invention provides methods and systems useful for developing in vitro an engineered tissue, method of using the tissue and compositions comprising the tissue. ... 11/30/06 - 20060270031 - Cell separation method and kit using phase separation A method of separating cells or viruses from a mixture using phase separation is provided. The method includes: suspending a sample containing cells or viruses in a kosmotropic salt solution having a pH of 3-6; aggregating the cells or viruses by adding a cationic polymer to the suspension; adhering the ... 11/30/06 - 20060270030 - Multimodally altered cells as a form for administering active substances and as diagnostic particles The present application demonstrates that both active substances and diagnostically effective substances can be introduced into biological cells, in particular blood cells, simultaneously. These multimodally altered cells can be found once again, in vivo and in vitro, using modern imaging methods. In addition to the diagnostic and active compound-carrier principles, ... 11/30/06 - 20060270029 - Automatable artificial immune system (ais) The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates ... 11/23/06 - 20060263876 - Neural crest stem cells and uses thereof This present invention features methods and composition for the isolation and proliferation of neural crest stem cells (NCSCs) from embryonic tissues as well as from tissues from a post-natal mammal. According to this invention, NCSCs are capable of producing non-neuronal and neuronal cells under the appropriate conditions. The cells of ... 11/16/06 - 20060258001 - Skin substitutes with improved purity The present invention relates to in vitro cultured skin substitutes. In particular, the present invention relates to compositions and methods for the development of cultured skin substitutes using NIKS cells. ... 11/16/06 - 20060258000 - Use of steady-state oxygen gradients to modulate animal cell functions The disclosure provides a bioreactor that allows steady-state oxygen gradients to be imposed upon in vitro culture systems. The bioreactor system of the disclosure has been applied to liver zonation and have shown that physiological oxygen gradients contribute to heterogeneity of tissue cultures in vitro. ... 11/09/06 - 20060252147 - Method of identifying target cell using target cell-specific virus Provided is a method of identifying a target cell using a virus. The method includes infecting the target cell with the virus by contacting the virus to the target cell and culturing the target cell to propagate the virus; adding a chromogenic substrate to the resultant cell culture to induce ... 10/26/06 - 20060240550 - Three-dimensional cell to tissue development process The present invention relates to an improved three-dimensional cell to tissue development process using a specific time varying electromagnetic force, pulsed, square wave, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar ... 10/19/06 - 20060234374 - Malignant cells and method for selecting the same The present invention is a method for selecting an enriched population of malignant cells. It has been found that by depleting fibroblasts from a population of cells obtained from a tissue sample; selecting epithelial cells from the fibroblast-depleted population; and culturing the selected epithelial cell population in the presence of ... 10/19/06 - 20060234373 - Treatment for diabetes Proliferating pancreatic islet cells obtained by the method of isolating a population of cells that preferably includes predominantly islet precursor cells that express one or more marker associated with an islet precursor cell and providing the precursor cells with one or more a pancreatic differentiation agent so that a population ... 10/12/06 - 20060228796 - Adipose tissue-derived adult stem cells for the repair of articular cartilage fractures and uses thereof The invention provides cells, methods and compositions based upon the use of adipose tissue-derived adult stem cells in the repair of articular cartilage fractures or defects. The invention is useful in providing a treatment of articular cartilage fractures in a clinical setting. ... 10/12/06 - 20060228795 - Apparatus for enhancing proliferation of cells in a small-scale cell culturing container An interchangeable sleeve for enhancing proliferation of cells in a small-scale culturing container is disclosed with the interchangeable sleeve having a substantially cylindrical and substantially rigid electrical conductive material wound in a cylindrical shape and capable of being connected to a pulsating time varying electromagnetic current to create a time ... 10/05/06 - 20060223178 - Devices and methods for magnetic enrichment of cells and other particles The invention features devices and methods for the enrichment of cells and other desired analytes by employing a magnetic field, alone or in conjunction with size-based separation. The devices and methods may be advantageously employed to enrich for rare cells, e.g., fetal cells or epithelial cells, present in a sample, ... 10/05/06 - 20060223177 - Postpartum cells derived from umbilical cord tissue, and methods of making and using the same Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described. ... 10/05/06 - 20060223176 - Integrated system for on-site cell acquisition, processing, and delivery Numerous embodiments of a system and method for treating cardiac tissue are described. In one embodiment, bone marrow cells are extracted from a patient. The cells are then processed to isolate mononuclear cells, which can then be delivered back near the cardiac tissue of the patient. ... 09/28/06 - 20060216820 - Use of retinoic esters of hyaluronic acid for the differentiation of totipotent stem cells The present the invention relates to a new use of retinoic of hyaluronic acid, that exhibit pre-differentiating activity on totipotent stem cells. The invention also relates to a process to differentiate said stem cells and to select molecules capable of modulating the pro-differentiating activity of those esters. ... 09/14/06 - 20060205068 - Novel bt toxin receptors from lepidopteran insects and methods of use The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins. ... 08/31/06 - 20060194314 - Novel bt toxin receptors from lepidopteran insects and methods of use The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins. ... 08/31/06 - 20060194313 - Shear protectants in harvest microfiltration The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration. ... 08/31/06 - 20060194312 - Method of cell culture observation, carbon substrate for cell culture observation, and method for manufacture thereof The invention provides a substrate suitable for cell culture observation and a method of observation using the same. Crystalline carbon such as a graphite powder is mixed into a thermosetting resin such as a furan resin, and the mixture is molded in the shape of a sheet and carbonized to ... 08/17/06 - 20060183220 - Automated cell sample enrichment preparation method A method for automated cell enrichment preparation where biological cells are injected into a first subchamber and flowed through the first subchamber, through a large pore filter and into a second subchamber so that the large pore filter retains particles large particles in the first subchamber, while the biological cells ... 08/10/06 - 20060177924 - Seeding cells on porous supports The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability. ... 08/10/06 - 20060177923 - Spatially-defined modification of fresh tissue using covalent chemistry Methods for modification of tissue using covalent chemistry. Tissue can be modified through direct alkylation, reduction followed by alkylation, or oxidation followed by condensation to covalently attach small organic molecules or appropriately modified proteins. The modification can be spatially limited to desired regions of the tissue surface. ... 07/20/06 - 20060160213 - Enhancement of mammalian embryo development A method of enhancing in vitro development of a mammalian embryo is disclosed which comprises supplementing the culture medium with a prostaglandin, or a prostaglandin analog, in an amount effective to promote complete hatching of the embryo (i.e., freeing of the embryo from the zona pellucida). The quality of human ... 07/20/06 - 20060160212 - Process for isolating haemocyanin A process for obtaining a substantially pure, stable solution of a molluscan haemocyanin, comprising the steps of: (1) collecting blood from a mollusc; (2) centrifuging the collected blood to remove cellular material and other particulates to obtain a serum fraction; (3) diafiltering the serum fraction to replace serum liquid with ... 07/13/06 - 20060154364 - Stem cell cultivation devices and methods The present invention relates to a device and methods for culturing stem cells, and in particular, for culturing ocular stem cells and the use of stem cells cultured using the devices and methods of the invention for the treatment of diseases. ... 07/06/06 - 20060148075 - Compositions, methods and systems for preparation of a stem cell-enriched cell population In accordance with some preferred embodiments, without limitation, the present invention comprises compositions, methods and systems for preparation of stem-cell enriched cell populations from sources of biological materials by sorting cell types in relation to size. ... 07/06/06 - 20060148074 - Serum-free mammalian cell culture medium, and uses thereof The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or ... 06/29/06 - 20060141619 - Apparatus and method for machining microchamber for cell culture There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, ... 06/29/06 - 20060141618 - Cell separation apparatus A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the ... 06/29/06 - 20060141617 - Multilayered microcultures A multilayer microculture capable of modeling complex in vitro structures such as mammalian tissues and organ structures is provided, along with methods for producing such a microculture and methods of using such microcultures for assaying for modulators of cell-cell interaction, cell migration, cell proliferation, cell adhesion or cellular or organismal ... 06/22/06 - 20060134779 - Modulation of cell intrinsic strain to control cell modulus, matrix synthesis, secretion, organization, material properties and remodeling of tissue engineered constructs The present invention provides methods for manipulating the intrinsic strain of cells by treating tissue engineered constructs or native tissue with compounds which affect the intrinsic strain setpoint of the cells in order to modulate matrix synthesis, secretion, organization and/or remodeling so that the tissues withstand in vivo mechanical forces ... 06/15/06 - 20060128011 - Replication of hepatitis c virus in non-hepatic epithelial and mouse hepatic cells Cells and cell lines which replicate HCV of non-hepatic human and non human origin are disclosed. Also provided are methods of using such cells and cell lines to identify anti-HCV agents for the treatment of HCV infection. ... 06/15/06 - 20060128010 - Method for preparing engineered tissue A method for preparing a human or animal tissue by applying a compressive force to a stack of sheets of living tissue thereby inducing adjacent layers to fuse or adhere to each other. The force is applied in direction normal to the surface of the tissue. A multi-layer tissue produced ... 06/08/06 - 20060121606 - Cell culture method and cultured tissue A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a ... 06/08/06 - 20060121605 - Selection and propagation of progenitor cells The invention relates to a population of progenitor cells and methods for obtaining and culturing the progenitor cells. Methods and compositions of the present invention can be useful in fields including regenerative medicine (tissue regeneration), transplantation, and cancer research. ... 06/01/06 - 20060115895 - Fish disease models and uses thereof Generation and use of disease models, for instance in assays to identify and investigate genes and substances involved in disease and disease treatment, identification and use of drug targets. In particular, the present invention relates to generation and use of disease models in fish such as zebrafish. Gradable phenotypes may ... 06/01/06 - 20060115894 - Method of and apparatus for facilitating processes of mammalian cells A method of facilitating processes of mammalian cells such as at least one of attachment, movement, growth, proliferation and differentiation comprises: supplying liquid comprising biologically compatible polymer to a liquid outlet in the vicinity of a surface and subjecting liquid issuing from the outlet to an electric field to cause ... 05/18/06 - 20060105456 - Method for purifying adenoviruses Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by ... 05/18/06 - 20060105455 - Covalently attached collagen vi for cell attachment and proliferation Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, ... 05/18/06 - 20060105454 - Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be ... 05/18/06 - 20060105453 - Coating process for microfluidic sample arrays A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one ... 05/18/06 - 20060105452 - Convective flow tissue assembly The present invention provides for an improved in vitro tissue assembly system and related methods that includes and uses a bioreactor, a porous mandrel disposed in the bioreactor, and components that provide for the circulation of culture media and cell suspensions within the bioreactor and through the porous mandrel. The ... 05/11/06 - 20060099707 - Automated cell preparation system and method An apparatus and method for automated cell preparation is described. A biological cell sample, including large particles and smaller objects of interest, is introduced into a first chamber. The large particles are trapped in the first chamber using a first filter, while smaller objects-of-interest and small particles pass through the ... 05/04/06 - 20060094111 - Dna containing variant frt sequences To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene ... 05/04/06 - 20060094110 - Use of spliceosome mediated rna trans-splicing for immunotherapy Methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated trans-splicing that result in expression of an immunogenic polypeptide. The invention includes pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of ... 04/20/06 - 20060084167 - Formation of hybrid cells by fusion of lineage committed cells with stem cells The potential of a stem cell to differentiate into specialized cell types for restoring normal tissue/organ function has stimulated interest in stem cell research. The methods used to coax stem cells differentiate into specialized cells still remain in their infancy stages. The disclosed invention is the generation of mammalian or ... 04/13/06 - 20060078989 - Dedifferentiated, programmable stem cells of monocytic origin, and their production and use The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for ... 04/06/06 - 20060073587 - Neuronal progenitors from feeder-free human embryonic stem cell culture The present invention relates to methods for producing feeder cell-free neuroprogenitor cells (preferably adherent) from embryonic stems cells, preferably human embryonic stem cells, the feeder cell-free neuroprogenitor cells, preferably human cells themselves, as well as methods for producing feeder cell-free samples of neuronal cells, preferably adherent human neuronal cells and ... 03/30/06 - 20060068494 - Wnt4 in supporting lymphopoiesis The T lineage differentiative potential of lymphoid progenitor cells in the lymph nodes is thwarted by the absence of Wnt signaling, resulting in blockade of the DN1→DN2 transition with accumulation of pre-DN2 cells. Wnt4 transcripts are deficient in the lymph nodes relative to the thymus. When cultured with stromal cells ... 03/23/06 - 20060063253 - Compositions, methods and kits relating to thrombin, notch signaling and stamatogenesis and growth of stem cells The present invention relates to methods based on the interactions of thrombin as a biological regulator. More specifically, the invention relates to the interactions of thrombin with regard to Notch signaling, Jagged1, PAR1, and cellular effects mediated thereby. The invention relates to the discovery that thrombin cleaves Jagged1 to produce ... 03/16/06 - 20060057715 - Methods and kits for isolating sperm cells Disclosed are methods for isolating sperm cells from an aqueous sample and kits for isolating sperm cells from an aqueous sample. ... 03/09/06 - 20060051861 - Cell lines for use in increasing protein yield from a cell culture The present invention provides cell lines useful in the production proteins and peptides. The cell lines contain recombinant expression constructs. The recombinant expression construct encodes the STPs consisting of the Cy protein motif and/or an ankyrin-binding protein motif. Each recombinant expression construct also contains an inducible transcription regulation element having ... 03/09/06 - 20060051860 - Method of organ regeneration It is intended to provide a method of regenerating an organ or a part thereof which comprises transplanting bone marrow or hematopoietic stem cells into an injured mammal or a mammal having an injured in organ or a part thereof; a method of treating injury: a method of producing an ... 02/16/06 - 20060035372 - Stem-like cells A method for the production and use of multipotential stem-like cells is disclosed. The preparation utilized in this method is characterized by the contact of low level electrical currents with cultures of fibroblasts or other -blast cells enriched by fibroblast growth factor and other nutrients. The electrical current is conducted ... 02/09/06 - 20060030040 - Human embryonic stem cells and culturing methods thereof Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ... 02/09/06 - 20060030039 - Placental stem cell and methods thereof The present invention describes stem cells obtained from post-partum placenta and their methods of obtaining and culturing. The present invention also describes compositions comprising placental stem cells and methods of using placental stem cells. ... 02/02/06 - 20060024823 - Cell culture carriers, method for manufacturing cell culture carriers and method for culturing cells Cell culture carriers that allow cells to efficiently adhere thereto and sufficiently grow thereon and from which the grown cells can be easily removed or detached, a method for manufacturing cell culture carriers that enables to easily and reliably manufacture such cell culture carriers and a method for culturing cells ... 02/02/06 - 20060024822 - System and method for cultivating cells The present invention discloses a system for cultivating cells, wherein the system comprises a bioreactor, a pump, and an electromagnetic module comprising a coil and an electromagnetic stimulator. The cells are disposed within the bioreactor. The pump, connected to the bioreactor, is used to drive gas into the bioreactor, so ... 01/26/06 - 20060019383 - Chemical inhibitors of mismatch repair Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of ... 01/19/06 - 20060014277 - Novel human kielin-like proteins and polynucleotides encoding the same Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications. ... 01/12/06 - 20060008900 - Pigment epithelium-derived factor: characterization, genomic organization and sequence of the pedf gene Nucleic acids encoding the neurotrophic protein known as pigment epithelium-derived factor (PEDF), a truncated version of PEDF referred to as rPEDF, and equivalent proteins, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing PEDF, rPEDF, and equivalent proteins, the rPEDF protein ... 01/05/06 - 20060003444 - Cells for detection of influenza and parainfluenza viruses The invention provides cell lines which are useful for the rapid detection and production of influenza and parainfluenza viruses. In particular, the invention relates to transgenic mink lung cells which show increased sensitivity to infection by influenza A, influenza B, or parainfluenza 3 viruses, or which are capable of enhanced ... 01/05/06 - 20060003443 - Recombinase-expressing cells In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in ... 01/05/06 - 20060003442 - Cell culture scaffold, methods of manufacturing the scaffold and cell culture methods A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised ... 01/05/06 - 20060003441 - Method for cultivating cells, particularly human or animal cells The invention relates to a method for cultivating cells of the most diverse type, particularly human or animal cells. According to the invention, a culture is prepared from cells of at least one specified type in a defined environment, and the cells of the relevant culture are supplied with assigned, ... 12/15/05 - 20050277189 - Method for stimulating self-renewal of neural stem cells and enhancing neurogenesis The present invention provides a mechanism for obtaining an almost unlimited source of stem cells. A method for the expansion of a population of stem cells that retain their developmental capacity comprises co-culture of a stem cell population with a trophic support, for example, endothelial cells or conditioned medium derived ... 12/01/05 - 20050266551 - Method for the controlled release of calcium from intracellular calcium stores of oocytes A method for the controlled release of calcium from intracellular calcium stores of oocytes, by i) establishing an optimum profile for release of calcium from intracellular calcium stores of oocytes, and ii) applying the following cycles to the oocytes: (A) a pulse perfusion cycle which comprises a step of perfusing ... 10/20/05 - 20050233442 - Carrier for cell culture A carrier for cell culture having multi-layer structure comprising a water-containing polymer gel layer, wherein the water-containing polymer gel layer has a thickness of 0.01 μm or more and 5 μm or less is provided. The carrier for cell culture preferably comprises a gel layer comprising an anionic polysaccharide and ... 09/22/05 - 20050208650 - Application apparatus of 3-dimensional klinostat and method of growing object using the same In order to grow an object to be cultivated or grown, a growth object is encapsulated in a vessel, and the growth object is grown without substantial influence of gravity. ... 08/18/05 - 20050181500 - Novel human uncoupling proteins and polynucleotides encoding the same The present invention relates to methods and compositions for the treatment of biological disorders regulatable by the controlled expression or inhibition of the described NUCPs. ... 08/11/05 - 20050176139 - Placental stem cell and methods thereof The present invention describes stem cells obtained from post-partum placenta and their methods of obtaining and culturing. The present invention also describes compositions comprising placental stem cells and methods of using placental stem cells. ... 06/30/05 - 20050142658 - Chromosomal saturation mutagenesis In One aspect, the invention provides methods for Chromosomal Saturation Mutagenesis (CSM) comprising generating randomly mutated, overlapping segments for an entire chromosome using error-prone PCR or other techniques, and libraries of nucleic acids made by these methods. ... 06/16/05 - 20050130298 - Hek293 cell line for activity studies and hts system of alpha1g t-type ca2+ channel There is provided a cell line that is prepared by transforming HEK293 cell with a human Kir2.1 gene using a retrovirus expression system, wherein the HEK293 expresses a stable α1G T-type calcium channel. The cell line responds sensitively to KCl and forms an appropriate level of the membrane voltage so ... 06/02/05 - 20050118710 - Methods and compositions for defining gene function A process for producing and analyzing insertionally mutated cell clones is provided. Cells incorporating insertional mutations are provided. A collection of insertionally mutated cell clones is also provided. ... ### FreshPatents.com Support - Terms & Conditions |